Mouse LIGHT, His Tag, E. coli

Catalog Number

LDG078PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 20.92 kDa.
    The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce cytotoxicity in HT-29 cells. The ED₅₀ for this effect is <2 μg/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs). Mouse LIGHT shares 79% sequence homology with human LIGHT. LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM). Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presencing anti-CD3 antibody treatment. Additionally, LIGHT is able to stimulate T cell proliferation and IFN expression via interacting with TNFRS13 /HVEM.

    Synonyms

    Tumor necrosis factor ligand superfamily member 14

  • Uniprot ID

    #Q9QYH9

    Sequence Note

    Arg58-Val239

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse LIGHT.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.