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Leadgene Leadgene
Mouse IL-3, His Tag, E. coli
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Catalog Number

LDG026PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.04 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce NFS-60 cells proliferation. The ED₅₀ for this effect is <85 pg/mL. The specific activity of recombinant mouse IL-3 is approximately >1x 10⁷ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3 also enhances phagocytosis and antibody-mediated cellular cytotoxicity. IL-3 binds to IL-3R (IL-3 receptor), which is composed of a unique α subunit (IL-3Rα) and a common β-subunit (βc).

      Synonyms

      Interleukin-3, Hematopoietic Growth Factors, Mast cell Growth Factors , MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

    • Uniprot ID

      #P01586

      Sequence Note

      Asp33-Cys166

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    The purity of recombinant mouse IL-3 is greater than 95% as determined by SEC-HPLC.

    The purity of recombinant mouse IL-3 is greater than 95% as determined by SEC-HPLC.

    SDS-PAGE analysis of recombinant mouse IL-3.

    SDS-PAGE analysis of recombinant mouse IL-3.

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  • Datasheet & Documents
    MSDS
    Manual
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