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Species of Origin
Mouse
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 18.41kDa.
The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to induce CXCL1 secretion in HT‑29 cells. The ED₅₀ for this effect is <1 ng/mL
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.
Synonyms
Interleukin-25, IL-25
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Uniprot ID
#NP_542767 1
Sequence Note
Val17-Ala169
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant mouse IL-25.]()
SDS-PAGE analysis of recombinant mouse IL-25.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
