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Leadgene Leadgene
Mouse GFAP, His Tag, E. coli
Catalog Number

LDG093PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 50.7 kDa.
      The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      GFAP (Glial Fibrillary Acidic Protein) is a protein primarily found in CNS astrocytes. Increased GFAP immunoreactivity indicates gliosis, a response to neural damage. GFAP defects cause Alexander disease, a rare CNS disorder with astrocytic Rosenthal fiber accumulation. The infantile form leads to myelination failure and early mortality, while the juvenile or adult forms present with ataxia, bulbar signs, spasticity, progressing more gradually.

      Synonyms

      Glial fibrillary acidic protein

    • Uniprot ID

      P03995-1

      Sequence Note

      Met1-Met430

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse GFAP protein.

    SDS-PAGE analysis of recombinant mouse GFAP protein.

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  • Datasheet & Documents
    MSDS
    Manual
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