Leadgene Leadgene
Mouse CD8 Alpha, His Tag, HEK293
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Catalog Number

LDG003PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      HEK293

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.69 kDa.
      The protein migrates as 30-35 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to support the adhesion of Jurkat human acute T cell leukemia cells, with the ED50 ranging from 0.750 to 5 μg/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD8, or cluster of differentiation 8, is a glycoprotein found on the surface of cytotoxic T cells, also known as CD8+ T cells. These cells are a vital component of the immune system, responsible for recognizing and destroying cells infected with viruses, bacteria, or cancerous cells. CD8 binds to major histocompatibility complex class I (MHC-I) molecules presenting antigenic peptides, initiating the activation of cytotoxic T cells. The presence of CD8 on the cell surface serves as a marker for these effector T cells, crucial for mounting an effective immune response against pathogens and tumors.

      Synonyms

      CD8a,T-cell surface glycoprotein Lyt-2

    • Uniprot ID

      P01731

      Sequence Note

      Met1-Tyr196

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Mouse CD8 Alpha, His Tag, HEK293. Measured by its ability to support the adhesion of Jurkat human acute T cell leukemia cells, with the ED50 ranging from 0.750 to 5 μg/mL.

    Activity analysis of recombinant Mouse CD8 Alpha, His Tag, HEK293.
    Measured by its ability to support the adhesion of Jurkat human acute T cell leukemia cells, with the ED50 ranging from 0.750 to 5 μg/mL.

    SDS-PAGE analysis of recombinant Mouse CD8 Alpha.

    SDS-PAGE analysis of recombinant Mouse CD8 Alpha.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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