Leadgene Leadgene
Mouse CD36, His Tag, CHO
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Catalog Number

LDG011PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      CHO

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 47.2 kDa.
      The protein migrates as 60-75 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to bind human R-Spondin 1 in functional ELISA. Immobilized Recombinant human R-Spondin 1 Protein at 10 μg/mL (100 μL/well) can bind Recombinant Mouse CD36 Protein.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD36, a transmembrane glycoprotein, is widely expressed on various cell types including macrophages, adipocytes, and endothelial cells. It serves as a multifunctional receptor involved in lipid metabolism, inflammation, and immune responses. CD36 facilitates the uptake of fatty acids, oxidized low-density lipoproteins (LDL), and other lipid molecules, contributing to cellular lipid homeostasis and foam cell formation in atherosclerosis. Additionally, CD36 recognizes and binds to a diverse range of ligands including microbial pathogens, apoptotic cells, and thrombospondin-1, mediating phagocytosis, innate immune responses, and tissue remodeling. Understanding CD36's diverse functions is crucial for elucidating its roles in health and disease.

      Synonyms

      Glycoprotein IIIb (GPIIIB), PAS IV, PAS-4, Platelet glycoprotein IV (GPIV)

    • Uniprot ID

      Q08857

      Sequence Note

      Gly30-Lys439

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Mouse CD36, His Tag, CHO. Measured by its ability to bind human R-Spondin 1 in functional ELISA. Immobilized Recombinant human R-Spondin 1 Protein at 10 μg/mL (100 μL/well) can bind Recombinant Mouse CD36 Protein.

    Activity analysis of recombinant Mouse CD36, His Tag, CHO.
    Measured by its ability to bind human R-Spondin 1 in functional ELISA. Immobilized Recombinant human R-Spondin 1 Protein at 10 μg/mL (100 μL/well) can bind Recombinant Mouse CD36 Protein.

    SDS-PAGE analysis of recombinant Mouse CD36.

    SDS-PAGE analysis of recombinant Mouse CD36.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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