Leadgene Leadgene
Mouse CD16A, His Tag, CHO
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Catalog Number

LDG100PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      CHO

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 21.47 kDa.
      The protein migrates as 20-33 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to bind mouse IgG in functional ELISA. Immobilized Recombinant mouse IgG at 1 μg/mL (100 μL/well) can bind Recombinant Mouse CD16A Protein.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD16A (FcγRIII) is an Fc receptor expressed on NK cells and macrophages, mediating antibody-dependent cellular cytotoxicity (ADCC).

      Synonyms

      Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-2, FcgammaRIV, CD16a

    • Uniprot ID

      A0A0B4J1G0

      Sequence Note

      Gly21-Gln203

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    HPLC analysis of recombinant Mouse CD16A, His Tag, CHO. The purity of Mouse CD16A Protein is greater than 95% as determined by RP-HPLC.

    HPLC analysis of recombinant Mouse CD16A, His Tag, CHO.
    The purity of Mouse CD16A Protein is greater than 95% as determined by RP-HPLC.

    SDS-PAGE analysis of recombinant Mouse CD16A.

    SDS-PAGE analysis of recombinant Mouse CD16A.

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  • Datasheet & Documents
    MSDS
    Manual
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