Mouse CD133, His Tag, HEK293

Catalog Number

LDG138PMM

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression System

    HEK293

  • Affinity Tag

    His Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 29.1 kDa.
    The protein migrates as 35-58 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    CD133 (Prominin-1) is a pentaspan transmembrane glycoprotein widely recognized as a marker of stem and progenitor cells, including hematopoietic, neural, epithelial, and cancer stem cells. CD133 plays key roles in maintaining stemness, regulating cell polarity, and promoting tumor initiation and progression. It is frequently used in cancer stem cell research, organoid development studies, and tumor microenvironment characterization. Recombinant CD133 expressed in HEK293 cells provides native-like folding and glycosylation for antibody screening and functional assays.

    Synonyms

    Prominin-1, Prom1, CD133 antigen, AC133 antigen, Stem cell marker CD133, Cancer stem cell marker CD133, Pentaspan membrane glycoprotein, Neural stem cell surface marker, Hematopoietic progenitor antigen, Tumor-initiating cell marker, PROM1_MOUSE (UniProt reference), AC133 cell surface antigen

  • Uniprot ID

    O54990

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant Mouse CD133.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.