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LDG034PMM

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Specifications
- Species of Origin
  
  Mouse
  
  Expression System
  
  CHO
- Affinity Tag
  
  His Tag (C-term)
  
  Storage Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 104.35 kDa.
  The protein migrates as 40-80 kDa under reducing condition (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to bind mouse HGF in functional ELISA. Immobilized Recombinant mouse HGF Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse c-Met Protein.
  
  Form
  
  Lyophilized
Background
- Background
  
  Mouse cMet, also known as the hepatocyte growth factor receptor (HGFR), is a receptor tyrosine kinase involved in various cellular processes, including growth, survival, and migration. cMet is particularly important in the context of cancer research, as it plays a key role in tumor development and metastasis. The Mouse cMet protein, when expressed in CHO (Chinese Hamster Ovary) cells, can be tagged with a His (histidine) tag, which simplifies its purification and detection. This recombinant Mouse cMet-His fusion protein is widely utilized in studies aiming to understand cMet signaling pathways and to develop targeted therapies against cancers that overexpress this receptor.
  
  Synonyms
  
  Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, cMet
- Uniprot ID
  
  GenBank: EDL13881.1
  
  Sequence Note
  
  Glu25-Asn929
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Activity analysis of recombinant Mouse c-Met, His Tag, CHO.
Measured by its ability to bind mouse HGF in functional ELISA. Immobilized Recombinant mouse HGF Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse c-Met Protein.

HPLC analysis of recombinant Mouse c-Met, His Tag, CHO.
The purity of Mouse c-Met Protein is greater than 95% as determined by SEC-HPLC.

SDS-PAGE analysis of recombinant Mouse cMet.

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Datasheet & Documents

Datasheet

MSDS

Manual

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