Mouse Beta-NGF, His Tag, E. coli

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Catalog Number

LDG090PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 14.41 kDa.
    The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse beta-NGF is > 1 x 10⁶ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Beta-Nerve Growth Factors (Beta-NGF) is a 27 kDa cytokine with 241 amino acid residues. Beta-NGF belongs to neurotrophin family, and acts as neurotrophic factors. It's composed of alpha, beta, gamma subnuits, and the beta subunit is related to its biological activity. Beta-NGF binds to p75 neurotrophin receptor and Trk receptor and their function is about cell death and survival, respectively.

    Synonyms

    Beta-nerve Growth Factors, Beta-NGF

  • Uniprot ID

    #P01139

    Sequence Note

    Ser122-Gly241

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  The purity of recombinant mouse Beta-NGF is greater than 95% as determined by RP-HPLC.

  SDS-PAGE analysis of recombinant mouse Beta-NGF.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.