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Leadgene Leadgene
Mouse Anti-Camelid VHH Antibody Cocktail (HRP)
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Catalog Number

LDG0054YE

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  • Overview

    Description

    VHH antibodies, also known as single-domain antibodies or nanobodies, are derived from the variable domains of camelid heavy-chain-only antibodies. Their small size, high stability, and strong antigen-binding capability make them useful in a wide range of research applications.

    Leadgene Mouse Anti-Camelid VHH Antibody Cocktail (HRP) is an HRP-conjugated secondary antibody composed of multiple mouse monoclonal antibodies against camelid VHH. It specifically recognizes VHH antibodies derived from camel and llama.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-10000
    WB: 1:2000-10000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications

    • Host

      Mouse

      Immunogen

      Purified Camelid VHH Antibody

    • Reactivity

      Camel

      Application

      ELISA, WB

    • Conjugation

      HRP

      Storage Buffer

      Phosphate Buffered Saline containing 1% BSA, 0.03% ProClin 300 and 10% glycerol, pH 7.4.

    • Specificity

      Camel VHH Antibody

      Form

      Liquid

  • Instruction

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles and protect from light.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Recognition coverage across 30 VHH antibodies. Using OD₄₅₀ > 1.0 as the positive cutoff, Leadgene achieved 100% coverage (30/30), compared with 33.3% (10/30) and 16.7% (5/30) for competitor antibodies.

    Recognition coverage across 30 VHH antibodies.
    Using OD₄₅₀ > 1.0 as the positive cutoff, Leadgene achieved 100% coverage (30/30), compared with 33.3% (10/30) and 16.7% (5/30) for competitor antibodies.

    Borad and consistent recognition of diverse VHH antibody. Figure A. ELISA dose–response curves across diverse VHH species. Leadgene demonstrated strong and specific binding to camelid, llama, and humanized VHH, with higher overall signals and lower apparent EC₅₀ values than competitor antibodies. Figure B. Recognition profile across 30 VHH antibodies. The heatmap shows broad and consistently stronger recognition by Leadgene compared with competitor antibodies. Figure C. Signal distribution across 30 VHH antibodies. The box plot summarizes the ELISA signals from 30 VHH antibodies, demonstrating significantly higher overall signals for Leadgene than for competitor antibodies.

    Borad and consistent recognition of diverse VHH antibody.

    Figure A. ELISA dose–response curves across diverse VHH species.
    Leadgene demonstrated strong and specific binding to camelid, llama, and humanized VHH, with higher overall signals and lower apparent EC₅₀ values than competitor antibodies.

    Figure B. Recognition profile across 30 VHH antibodies.
    The heatmap shows broad and consistently stronger recognition by Leadgene compared with competitor antibodies.

    Figure C. Signal distribution across 30 VHH antibodies.
    The box plot summarizes the ELISA signals from 30 VHH antibodies, demonstrating significantly higher overall signals for Leadgene than for competitor antibodies.

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  • Datasheet & Documents
    Datasheet
    MSDS
    Manual
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