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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>97% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 18.3 kDa.
The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).
Endotoxin Level
<0.05 EU per 1 µg of the protein by the LAL method.
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Mycoplasma
Not detected
Form
Lyophilized
More Images
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Specifications
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Background
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Background
Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.
Synonyms
Interleukin-7
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Uniprot ID
P13232
Sequence Note
Asp26-His177
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Manufacturing Specifications
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
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Image
![LeadGMP® Human IL-7 Protein, His Tag, E. coli (LDG007PHE-GMP) stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED50 < 0.8 ng/mL.]()
LeadGMP® Human IL-7 Protein, His Tag, E. coli (LDG007PHE-GMP) stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED50 < 0.8 ng/mL.
![SDS-PAGE analysis of LeadGMP® IL-7, Human]()
SDS-PAGE analysis of LeadGMP® IL-7, Human
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
