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Leadgene Leadgene
LeadGMP® Human IL-7 Protein, His Tag, E. coli
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Catalog Number

LDG007PHE-GMP

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >97% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 18.3 kDa.
      The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).

      Endotoxin Level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.

      Synonyms

      Interleukin-7

    • Uniprot ID

      P13232

      Sequence Note

      Asp26-His177

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing Specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    LeadGMP® Human IL-7 Protein, His Tag, E. coli (LDG007PHE-GMP) stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED50 &lt; 0.8 ng/mL.

    LeadGMP® Human IL-7 Protein, His Tag, E. coli (LDG007PHE-GMP) stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED50 < 0.8 ng/mL.

    SDS-PAGE analysis of LeadGMP® IL-7, Human

    SDS-PAGE analysis of LeadGMP® IL-7, Human

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  • Datasheet & Documents
    MSDS
    Manual
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