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LeadGMP® Human IFN Gamma Protein, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >97% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.7 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cytotoxicity in HT29 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human IFN gamma is approximately >1 x 10⁷ IU/mg, which is calibrated against the human IFN Gamma WHO Reference Material (NIBSC code: 87/586).

      Endotoxin Level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected



  • Background
    • Background

      The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.


      Interferon gamma, Immune interferon

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing Specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    LeadGMP® Human IFN Gamma Protein, His Tag, E. coli (LDG013PHE-GMP) induces cytotoxicity in HT29 cells, with the ED50 &lt;1.0 ng/mL.

    LeadGMP® Human IFN Gamma Protein, His Tag, E. coli (LDG013PHE-GMP) induces cytotoxicity in HT29 cells, with the ED50 <1.0 ng/mL.

    SDS-PAGE analysis of LeadGMP® IFN gamma, Human

    SDS-PAGE analysis of LeadGMP® IFN gamma, Human

    Total   (2)
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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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