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Leadgene Leadgene
LeadGMP® Human GM-CSF Protein, His Tag, E. coli
Catalog Number

LDG012PHE-GMP

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 15.4 kDa.
      The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.

      Endotoxin Level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a growth factor due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

      Synonyms

      Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor , CSF, Molgramostin, Sargramostim

    • Uniprot ID

      P04141

      Sequence Note

      Ala18-Glu144

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing Specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    SDS-PAGE analysis of LeadGMP® GM-CSF, Human

    SDS-PAGE analysis of LeadGMP® GM-CSF, Human

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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