LeadGMP® Human Activin A Protein, Tag Free, E. coli

Catalog Number

LDG026PHE-GMP

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    Tag Free

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >97% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 12.96 kDa.
    The protein migrates as 13-17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg.

    Endotoxin Level

    <0.05 EU per 1 µg of the protein by the LAL method.

  • Mycoplasma

    Not detected

    Form

    Lyophilized

• Background

  • Background

    Activin is the member of the TGF beta superfamily of cytokines. The current understanding of Activin A is classified as a hormone, a growth factor, and a cytokine. Depending on the different cell type, that overexpression of Activin A can either inhibit or promote cells division. There are important implications for tumor biology. Activin A also increase joint inflammation in rheumatoid arthritis (RA) and induces pathogenic mechanisms in the respiratory system.

    Synonyms

    Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein , EDF

  • Sequence Note

    Gly311-Ser426

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    Manufacturing Specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  SDS-PAGE analysis of LeadGMP® Activin A, Human

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.