Animal-free reagent and laboratory
Manufactured and tested under GMP guideline
MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
Interleukin-1 beta (IL-1β) is a major cytokine expressed in macrophage, NK cells, monocytes, and neutrophils. It also plays fundamental role in inflammatory response, including monocyte activation, which is essential for the host defense and pathogen and pathogen resistance. Pro-IL-1β is cleaved by cytosolic caspase 1 to form mature IL-1β.
Measure by its ability to induce proliferation in D10.G4.1 cells.
The ED50 for this effect is <10 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately >1.5 x 108 IU/mg.
Measure by its ability to induce IL-8 secretion in HT29 cells.
The ED50 for this effect is 1.8-5.1 ng/mL.
Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.
1. Yazdi AS, Ghoreschi K. (2016) Adv Exp Med Biol. 941: 21‐29.
2. Gabay C. et al. (2010) Nat Rev Rheumatol. 6,4: 232-41.
3. Lopez-Castejon G, Brough D. (2011) Cytokine Growth Factor Rev. 22,4: 189‐95
4. Weber A. et al. (2010) Sci Signal. 3,105: cm2.
*Carriers: 0.1% BSA, 5% HSA or 10% FBS.