LEADGENE® Human Irisin, Tag Free

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  • Catalog number
    63631 / 63632 / 63633
  • Package
    5 μg / 20 μg / 100 μg
  • Species
  • Endotoxin level
    <0.1 EU per 1 μg of the protein by the LAL method.

Escherichia coli


Lyophilized protein should be stored at -20°C.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

  1. Keep ampoules at -20°C or -80°C for storage.
  2. No needs to spin the ampoule before opening. Remove the cap carefully.
  3. Dissolve the lyophilized protein by adding sterile ddH2O along the inner side of ampoule to avoid generating bubbles. Make protein concentration to be at 100 μg/mL, or consult product’s Certificate of Analysis (CoA). (Note: No needs to add carriers* to the initial reconstitution solution). Wear gloves when manipulating the sample to prevent contamination from proteases that may degrade the reconstituted protein.
  4. Leave the reconstituted protein solution at room temperature for at least 20 minutes.
  5. Pipet the solution gently. The solution should be clear if proteins are fully dissolved. (Important Note: Do Not Vortex! Vigorous shaking may impair biological activity of protein.)
  6. Use the reconstituted solution immediately, or make aliquots and store at -20 °C. (Important Note: Avoid repeated freeze-thaw cycles)
  7. We recommend diluting the initial reconstitution solution to working concentration with buffers or medium containing carriers*. (Important Note: Do not use water for dilution, which may cause protein degradation.)

*Carriers: 0.1% BSA, 5% HSA or 10% FBS.

SDS-PAGE analysis of Human Irisin, Tag Free
SDS-PAGE analysis of Human Irisin, Tag Free

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LEADGENE® Human Irisin, Tag Free

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  • LEADGENE® Human Irisin, Tag Free
  • Spec

    63631 / 5 μg

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