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LEADGMP® IL-7 (Interleukin-7), Human

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  • Catalog number
    61201GMP / 61202GMP
  • Package
    100 μg / 1 mg
  • Species
    Human
  • Tag
    polyhistidine tag at the C-terminus
  • Endotoxin level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Formulation
    The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Escherichia coli
Animal-free reagent and laboratory
Manufactured and tested under GMP guideline

MDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH with polyhistidine tag at the C-terminus.

Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC).
The ED50 for this effect is <0.8 ng/mL.
The specific activity of recombinant human IL-7 is > 7 x 108 IU/mg

Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.

SDS-PAGE analysis of LeadGMP® IL-7, Human
SDS-PAGE analysis of LeadGMP® IL-7, Human

1. Lin J. et al. (2017) Anticancer Res. 37,3: 963-967.
2. Schluns K S. (2000) Nat Immunol. 1(5):426-32.
3. Ceredig R, Rolink AG (2012). Semin Immunol. 24,3: 159‐64.
4. Jiang Q. et al. (2005) Cytokine Growth Factor Rev.16,4-5: 513-33.

  1. Keep ampoules at -20°C or -80°C for storage.
  2. No needs to spin the ampoule before opening. Remove the cap carefully.
  3. Dissolve the lyophilized protein by adding sterile ddH2O along the inner side of ampoule to avoid generating bubbles. Make protein concentration to be at 100 μg/mL, or consult product’s Certificate of Analysis (CoA). (Note: No needs to add carriers* to the initial reconstitution solution). Wear gloves when manipulating the sample to prevent contamination from proteases that may degrade the reconstituted protein.
  4. Leave the reconstituted protein solution at room temperature for at least 20 minutes.
  5. Pipet the solution gently. The solution should be clear if proteins are fully dissolved. (Important Note: Do Not Vortex! Vigorous shaking may impair biological activity of protein.)
  6. Use the reconstituted solution immediately, or make aliquots and store at -20 °C. (Important Note: Avoid repeated freeze-thaw cycles)
  7. We recommend diluting the initial reconstitution solution to working concentration with buffers or medium containing carriers*. (Important Note: Do not use water for dilution, which may cause protein degradation.)

*Carriers: 0.1% BSA, 5% HSA or 10% FBS.

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LEADGMP® IL-7 (Interleukin-7), Human

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  • LEADGMP® IL-7 (Interleukin-7), Human
  • Spec

    61201GMP / 100 μg

  • Unit Price:

    US$1520

  • Qty:

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