Animal-free reagent and laboratory
Manufactured and tested under GMP guideline
MVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM with polyhistidine tag at the C-terminus.
Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.
Measure by its ability to induce proliferation in TF-1 cells.
The ED50 for this effect is <0.5 ng/mL.
The specific activity of recombinant human IL-6 is approximately >5 x 108 IU/mg.
Measure by its ability to induce proliferation in MCF-7 cells.
The ED50 for this effect is <4.4 ng/mL.
Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.
1. Tanaka T. et al. (2014) Cold Spring Harb Perspect Biol. 6,10: a016295.
2. Mihara M. et al. (2012) Clin Sci (Lond). 122,4: 143-59.
3. Kishimoto T. (2010) Int Immunol. 22,5: 347-52.
4. Tanaka T. et al. (2016) Adv Exp Med Biol. 941: 79-88.
*Carriers: 0.1% BSA, 5% HSA or 10% FBS.