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Leadgene Leadgene
LeadGMP® Human IL-3 Protein, His Tag, E. coli
Catalog Number

LDG004PHE-GMP

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16 kDa.
      The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.15 ng/mL. The specific activity of recombinant human IL-3 is approximately >1.2 x 10⁶ IU/mg.

      Endotoxin Level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3 also enhances phagocytosis and antibody-mediated cellular cytotoxicity. IL-3 binds to IL-3R (IL-3 receptor), which is composed of a unique α subunit (IL-3Rα) and a common β-subunit (βc).

      Synonyms

      Interleukin-3, Hematopoietic growth factor, Mast cell growth factor , MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

    • Uniprot ID

      P08700

      Sequence Note

      Ala20-Phe152

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing Specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    SDS-PAGE analysis of LeadGMP® IL-3, Human

    SDS-PAGE analysis of LeadGMP® IL-3, Human

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  • Datasheet & Documents
    MSDS
    Manual
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