LEADGMP^® IL-3 (Interleukin-3), Human

• Catalog number
  60801GMP / 60802GMP
• Package
  100 μg / 1 mg
• Species
  Human
• Tag
  polyhistidine tag at the C-terminus
• Endotoxin level
  <0.1 EU per 1 μg of the protein by the LAL method.
• Formulation
  The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Escherichia coli
Animal-free reagent and laboratory
Manufactured and tested under GMP guideline

Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3 also enhances phagocytosis and antibody-mediated cellular cytotoxicity. IL-3 binds to IL-3R (IL-3 receptor), which is composed of a unique α subunit (IL-3Rα) and a common β-subunit (βc).

Measure by its ability to induce TF-1 cells proliferation.
The ED₅₀ for this effect is <0.15 ng/mL.
The specific activity of recombinant human IL-3 is approximately >1.2 x 10⁶ IU/mg.

Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.

1. Wagemaker G et al. (1990) Biotherapy. 2,4: 337-45.
2. Guthridge MA. et al. (1998) Stem Cells. 16,5: 301-13.
3. Ihle JN. (1992) Chem Immunol. 51: 65-106.
4. Dougan M. et al. (2019) Immunity. 50,4: 796-811.

1. Keep ampoules at -20°C or -80°C for storage.
2. No needs to spin the ampoule before opening. Remove the cap carefully.
3. Dissolve the lyophilized protein by adding sterile ddH₂O along the inner side of ampoule to avoid generating bubbles. Make protein concentration to be at 100 μg/mL, or consult product’s Certificate of Analysis (CoA). (Note: No needs to add carriers* to the initial reconstitution solution). Wear gloves when manipulating the sample to prevent contamination from proteases that may degrade the reconstituted protein.
4. Leave the reconstituted protein solution at room temperature for at least 20 minutes.
5. Pipet the solution gently. The solution should be clear if proteins are fully dissolved. (Important Note: Do Not Vortex! Vigorous shaking may impair biological activity of protein.)
6. Use the reconstituted solution immediately, or make aliquots and store at -20 °C. (Important Note: Avoid repeated freeze-thaw cycles)
7. We recommend diluting the initial reconstitution solution to working concentration with buffers or medium containing carriers*. (Important Note: Do not use water for dilution, which may cause protein degradation.)

*Carriers: 0.1% BSA, 5% HSA or 10% FBS.