LEADGENE^® Influenza A H7N9 (A/Shanghai/02/2013) Nucleocapsid Protein, His Tag, E. coli

A DNA sequence encoding Influenza A H7N9 (SH) Nucleocapsid Protein was
expressed with polyhistidine tag at the N-terminus

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots
should be stored at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

1. Keep ampoules at -20°C or -80°C for storage.
2. No needs to spin the ampoule before opening. Remove the cap carefully.
3. Dissolve the lyophilized protein by adding sterile ddH₂O along the inner side of ampoule to avoid generating bubbles. Make protein concentration to be at 100 μg/mL, or consult product’s Certificate of Analysis (CoA). (Note: No needs to add carriers* to the initial reconstitution solution). Wear gloves when manipulating the sample to prevent contamination from proteases that may degrade the reconstituted protein.
4. Leave the reconstituted protein solution at room temperature for at least 20 minutes.
5. Pipet the solution gently. The solution should be clear if proteins are fully dissolved. (Important Note: Do Not Vortex! Vigorous shaking may impair biological activity of protein.)
6. Use the reconstituted solution immediately, or make aliquots and store at -20 °C. (Important Note: Avoid repeated freeze-thaw cycles)
7. We recommend diluting the initial reconstitution solution to working concentration with buffers or medium containing carriers*. (Important Note: Do not use water for dilution, which may cause protein degradation.)

*Carriers: 0.1% BSA, 5% HSA or 10% FBS.