產品資訊

LEADGENE® HRV 3C Protease

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  • Catalog number
    62530 / 62531 / 62532 HRV 3C Protease

    62530-0 / 62530-1 10X HRV 3C Cleavage Buffer

  • Package
    Bulk / 1,000 U / 10,000 U HRV 3C Protease

    Bulk / 10 mL 10X HRV 3C Cleavage Buffer

  • Tag
    polyhistidine tag
  • Endotoxin level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Formulation
    The protein was lyophilized from a solution containing 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.04% Tween20, 8% trehalose, 8% mannitol.

Escherichia coli

HRV 3C Protease is a recombinant form of the 3C protease derived from human rhinovirus 14 expressed in E. coli (specific activity 1800-2000 U/mg). This product is a highly purified recombinant 6XHis-fusion protein. This protease requires neither metal nor cofactors for activity. HRV 3C Protease recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP).

One unit of HRV 3C Protease is defined as the amount of enzyme that will cleave>95% of 0.1 mg HRV 3C cleavage control protein in 150 mM NaCl, 50 mM Tris-HCl pH 7.5, at 4°C for 16 h.

>98% as determined by SDS-PAGE.
Purified by Ni-NTA chromatography.

Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.

  1. 重組蛋白在未開封情況下可保存於-20°C或-80°C。
  2. 回溶前無須將安瓶離心,只需小心打開安瓶。將滅菌水小心加入安瓶,使蛋白濃度成為100 μg/mL,也可參考Certificate of Analysis (CoA)建議加入之體積。(注意:初始回溶時不建議使用緩衝液。)
  3. 小心沖洗管壁,盡量使附著於管內之蛋白皆回溶。室溫下靜置20分鐘。(特別注意:不可劇烈搖晃瓶身)
  4. 回溶後之蛋白可立刻使用或分裝凍於-20 °C,避免重複冷凍解凍。
  5. 若需要進一步稀釋使用,建議使用含有1% BSA、5% HAS或10% FBS之緩衝液進行稀釋。

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LEADGENE® HRV 3C Protease

產品詢價
  • LEADGENE® HRV 3C Protease
  • Spec

    62531 / 1000U

  • Unit Price:

    US$

  • Qty:

    +

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