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A typical sandwich ELISA involve the following procedures:

(1) The wells in a microtiter plate are coated with capturing antibodies.
(2) Samples and standards of known concentrations are added into the pre-coated wells.
(3) Wells are washed, and the detection antibody conjugated to Horse Radish Peroxidase (HRP) is applied to the plate to bind on the antigen.
(4) The plate is washed to remove excessive antibodies.
(5) Substrate is applied and converted by the enzyme HRP to elicit a chromogenic signal.
(6) The signal is then quantified by a spectrometer or some other optical device.

arigo guarantee to provide quality ELISA kits for your research needs.

arigo carefully selects the antibody pairs that give the best compatibility for optimal performance in ELISA assays.
We guarantee the reproducibility of our kits by ensuring that the CV values for intra- and inter assay precision of each kit to fall below 9% across all standard curves.
The sensitivity and specificity is carefully measured and documented for each kit. With the linear regression of >0.99 for each standard curves being plotted, we offer reliable kits to ensure that you get the optimal results for all of your experiments.
To help you perform your experiments in a more effective way, we have prepared a systematic one-page protocol for your convenience. Our phDs are always ready to answer any technical enquiries that you might have regarding our products.

♦ Human IFN alpha ELISA kit

arigo Company R Company B Company C
Sensitivity 6 pg/ml 12.5 pg/ml 1400 pg/ml 600 pg/ml
Assay Range 10.94-700 pg/ml 156-5000 pg/ml 3.15–200 ng/ml 0.6-400 ng/ml
Samples Serum, plasma, cell culture supernatant not reported Serum, plasma, cell culture supernatant Serum, plasma, cell culture supernatant