Description
IdeS Protease is a highly specific cysteine protease derived from Streptococcus pyogenes that cleaves human IgG antibodies at a single site in the lower hinge region. This enzyme is essential for therapeutic antibody and Fc fusion protein characterization, enabling the rapid generation of homogenous F(ab')2 and Fc fragments for detailed analysis via mass spectrometry or chromatography.
Expression System
Escherichia coli
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of 50 mM Sodium phosphate, 150 mM NaCl, pH 6.6.
Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 36.22 kDa.
The protein migrates as 32-35 kDa under reducing condition (SDS-PAGE analysis).
Activity
70 U/µL
Unit Definition
One unit is defined as the amount of enzyme required to cleave ≥ 95% of 1 µg recombinant human IgG in 30 minutes at 37°C.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
Synonyms
IgG-degrading enzyme of Streptococcus pyogenes, Endopeptidase IdeS, IgG-specific cysteine protease, Fab/Fc cleaving protease
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O and incubate the stock solution for at least 10 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at -20°C for 12 months in lyophilized state from date of receipt. Upon reconstitution, protein aliquots should be stored at 4°C.
SDS-PAGE analysis of recombinant human IgG digested with IdeS protease.
The standard assay was performed by incubating 2 units of IdeS protease and 2 μg of recombinant human IgG at 37°C for 30 minutes.
SDS-PAGE analysis of IdeS Protease.
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Disclaimer：For Research Use or Further Manufacturing Only.
