Description
The HyLink™ BirA Biotin Labeling Kit for Avi-tag Proteins provides a highly efficient, specific, and reproducible solution for biotinylating recombinant proteins or peptides containing Avi-tags or other enzymatic biotinylation tags such as Biotag™. This kit utilizes the biotin ligase enzyme BirA, which specifically and uniformly attaches biotin to a lysine residue within the Avi-tag sequence. Compared to traditional chemical biotinylation methods, this enzymatic approach offers site-specific labeling, minimizes variability between batches, and preserves the biological activity and structural integrity of the target protein.
Components
GST-BirA (lyophilized)
Quantity
40 μg, 1 vial
Storage
Store at -20°C
Reconstitution buffer
Quantity
0.2 mL, 1 vial
Storage
Store at -20°C
10X Reaction buffer
Quantity
1.5 mL, 1 vial
Storage
Store at -20°C
SpinDesalt Column
Quantity
16 pcs
Storage
Store at 4°C
