Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 20.11 kDa.
The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce HUVEC cells proliferation. The ED₅₀ for this effect is <5 ng/mL. The specific activity of recombinant human VEGF165 is approximately >1.4 x 10⁶ IU/mg.
<0.1 EU per 1 μg of the protein by the LAL method.
Vascular Endothelial Growth Factors 165(VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Human VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. Human VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.
Vascular permeability factor, VPF, Vascular endothelial Growth Factors A
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human VEGF165, His Tag, E. coli (LDG168PHE) induced HUVEC cell proliferation, with the ED50 at 4.51 ng/mL.
SDS-PAGE analysis of recombinant human VEGF165.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.