Leadgene Leadgene
Human VEGF165, His Tag, E. coli
More Images
Catalog Number


More Documents
Loading package & price...
  • Specifications
    • Species of Origin


      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.11 kDa.
      The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce HUVEC cells proliferation. The ED₅₀ for this effect is <12 ng/mL. The specific activity of recombinant human VEGF165 is approximately >5 x 10⁵ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Vascular Endothelial Growth Factors 165(VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Human VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. Human VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.


      Vascular permeability factor, VPF, Vascular endothelial Growth Factors A

    • Uniprot ID

      # NP_001165097

      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human VEGF165, His Tag, E. coli (LDG168PHE) induced HUVEC cell proliferation, with the ED50 at 4.51 ng/mL.

    Human VEGF165, His Tag, E. coli (LDG168PHE) induced HUVEC cell proliferation, with the ED50 at 4.51 ng/mL.

    SDS-PAGE analysis of recombinant human VEGF165.

    SDS-PAGE analysis of recombinant human VEGF165.

    Total   (2)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.