Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
>95% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 15.00 kDa.
The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce proliferation in HUVEC cells. The ED₅₀ for this effect is <2.5 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Vascular Endothelial Growth Factors 121(VEGF121) is one of five VEGF splice variants of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF121 is the only VEGF which lacks heparin-binding activity and freely diffusible. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1 /KDR) on endothelial cells to activate signal transduction and regulate both physiological and pathological angiogenesis.
Vascular endothelial Growth Factors A, VEGF-A, Vascular permeability factor, VPF
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human VEGF121, His Tag, E. coli (LDG060PHE) induced HUVEC cell proliferation, with the ED50 at 2.11 ng/mL.
SDS-PAGE analysis of recombinant human VEGF121.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.