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Leadgene Leadgene
Human TPO, His Tag, E. coli
Catalog Number

LDG065PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.6 kDa.
      The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.01 EU per 1 µg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Thrombopoietin (TPO) is a glycoprotein that produced by the liver, kidney, marrow stroma and several other tissues. The TPO level in the blood is mostly negatively correlated with the abundance of platelets and bone marrow megakaryocytes, although multiple states of inflammation or infection, liver failure, and hematological disturbances are associated with unexpectedly high or low circulating levels of the hormone.

      Synonyms

      Thrombopoietin, C-mpl ligand , ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor , MGDF, Myeloproliferative leukemia virus oncogene ligand

    • Uniprot ID

      #P40225

      Sequence Note

      Ser22-Leu195

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human TPO.

    SDS-PAGE analysis of recombinant human TPO.

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  • Datasheet & Documents
    MSDS
    Manual
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