Language
TW
Leadgene Leadgene
Human TGF Beta 3, His Tag, E. coli
More Images
Catalog Number

LDG152PHE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.66 kDa.
      The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is < 50 pg/mL. The specific activity of recombinant human TGF Beta 3 is approximately 2 x 10⁷ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution.

      Synonyms

      Transforming Growth Factors beta-3 proprotein, Latency-associated peptide, LAP, Transforming Growth Factors beta-3, TGF-beta-3

    • Uniprot ID

      # P10600 1

      Sequence Note

      Ala301-Ser412

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human TGF Beta 3, His Tag, E. coli (LDG152PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 43 pg/mL.

    Human TGF Beta 3, His Tag, E. coli (LDG152PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 43 pg/mL.

    SDS-PAGE analysis of recombinant human TGF beta 3.

    SDS-PAGE analysis of recombinant human TGF beta 3.

    1
    /
    2
    Total   (2)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.