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Leadgene Leadgene
Human TGF Beta 2, His Tag, E. coli
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Catalog Number

LDG091PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.66 kDa.
      The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF beta 2 is > 5 x 10⁶ IU/mg.

      Endotoxin Level

      <0.01 EU per 1 µg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Transforming Growth Factors beta 2 (TGFβ-2) is a 12.85 kDa member of the epidermal Growth Factors with 113 amino acid residues. TGFβ-2 is expressed from throughout the body. TGFβ-2 is a regulator of cell proliferation, differentiation, apoptosis, cell plasticity and migration, etc. TGF-β-2 also associates with various kinds of diseases, such as cancer and tissue fibrosis.

      Synonyms

      Latency-associated peptide, LAP, Transforming Growth Factors beta-2, TGF-beta-2, Glioblastoma-derived T-cell suppressor factor, Transforming Growth Factors beta-2 proprotein

    • Uniprot ID

      #P61812

      Sequence Note

      Ala303-Ser414

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human TGF Beta 2, His Tag, E. coli (LDG091PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 0.17 ng/mL.

    Human TGF Beta 2, His Tag, E. coli (LDG091PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 0.17 ng/mL.

    SDS-PAGE analysis of recombinant human TGF Beta 2.

    SDS-PAGE analysis of recombinant human TGF Beta 2.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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