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Leadgene Leadgene
Human TGF Alpha, His Tag, E. coli
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Catalog Number

LDG090PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 6.49 kDa.
      The protein migrates as 7 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 x 10⁶ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Transforming Growth Factors alpha (TGF-α) is a 5.68 kDa member of the epidermal Growth Factors with 51 amino acid residues. TGF-α is mainly expressed from brain, skin, epithelial cell (pancreatic endocrine cells, urothelial cells, oligodendrocyte precursor cells, etc.). TGF-α is a regulator of cell proliferation and differentiation via bind to the EGFR and to act synergistically with TGF β. TGF-α also associates with myriad forms of cancer.

      Synonyms

      EGF-like TGF, ETGF, TGF type 1, Protransforming Growth Factors alpha

    • Uniprot ID

      #P01135

      Sequence Note

      Trp40-Ala89

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human TGF Alpha, His Tag, E. coli (LDG090PHE) induced 3T3 cell proliferation, with the ED50 at 0.19 ng/mL.

    Human TGF Alpha, His Tag, E. coli (LDG090PHE) induced 3T3 cell proliferation, with the ED50 at 0.19 ng/mL.

    SDS-PAGE analysis of recombinant human TGF Alpha.

    SDS-PAGE analysis of recombinant human TGF Alpha.

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  • Datasheet & Documents
    MSDS
    Manual
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