Description
HSA is the most abundant protein in blood, playing a crucial role in maintaining colloid osmotic pressure, transporting various molecules (such as fatty acids, hormones, drugs, etc.), and exerting antioxidant effects. Furthermore, during the production of biopharmaceutical products, HSA may be introduced due to its widespread applications (for example, as a stabilizer and excipient). Monitoring and controlling the residual amount of HSA is essential for ensuring the safety, efficacy, and regulatory compliance of these products. Leadgene® Human serum albumin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of HSA level in sample solution. The Human serum albumin ELISA Kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Test Principle
Human serum albumin (HSA) ELISA Kit is used to detect HSA in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-HSA monoclonal antibody to the solid phase. HSA in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-HSA VHH antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of HSA level is accomplished by comparing the absorbance with standard curve.
