Human Sclerostin, His Tag, CHO

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Catalog Number

LDG055PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    CHO

  • Affinity Tag

    His Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >85% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 22.33 kDa.
    The protein migrates as 25-35 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <8 μg/mL.

    Form

    Lyophilized

• Background

  • Background

    Human Sclerostin is a glycoprotein primarily expressed by osteocytes and plays a key role in regulating bone formation by inhibiting the Wnt signaling pathway, which is crucial for bone growth. Sclerostin acts as a negative regulator of bone mass, and its activity is of significant interest in research related to osteoporosis and other bone-related disorders. The Human Sclerostin protein, when expressed in CHO (Chinese Hamster Ovary) cells, is often tagged with a His (histidine) tag, facilitating its purification and detection in research applications. This recombinant Sclerostin-His fusion protein is commonly used in studies aimed at understanding bone metabolism and developing therapeutic strategies for enhancing bone formation.

    Synonyms

    Sclerostin

  • Uniprot ID

    Q9BQB4

    Sequence Note

    Gln24-Tyr213

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human Sclerostin, His Tag, CHO.
  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <8 μg/mL.

  SDS-PAGE analysis of recombinant human Sclerostin.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.