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Leadgene Leadgene
Human R-Spondin 1, His-SUMO Tag, HEK293
Catalog Number

LDG013PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      HEK293 cell

    • Affinity Tag

      His-SUMO Tag (N-term)

      Concentration

      Please refer to the product label.

    • Storage Buffer

      Liquid. Phosphate buffered saline, pH 7.4.

      Purity

      >95% as determined by SDS-PAGE analysis.

    • Molecular weight

      The protein has a calculated MW of 46.8 kDa.
      The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).

      Activity

      R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

    • Endotoxin Level

      <1 EU per 1 μg of the protein by the LAL method.

      Form

      Liquid

  • Background
    • Background

      R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/ 6), subsequent clearing negative regulators ZNRF3 /RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.

      Synonyms

      Roof plate-specific spondin-1, hRspo1

    • Uniprot ID

      # Q2MKA7

      Sequence Note

      Ser21-Ala263

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long-term storage.

      Stability & Storage

      This product is stable after storage at:

      • -80°C for long-term storage under sterile conditions.

      Avoid repeated free-thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human R-Spondin 1.

    SDS-PAGE analysis of recombinant human R-Spondin 1.

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  • Datasheet & Documents
    MSDS
    Manual
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