Leadgene Leadgene
Human Plasma kallikrein, His Tag, CHO
Catalog Number

LDG053PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      CHO

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 72.62 kDa.
      The protein migrates as 75 kDa under reducing condition (SDS-PAGE analysis).

    • Form

      Lyophilized

  • Background
    • Background

      Human Plasma Kallikrein is a serine protease involved in the kallikrein-kinin system, playing a crucial role in blood coagulation, inflammation, and blood pressure regulation. It is activated from its precursor, prekallikrein, and contributes to the generation of bradykinin, a potent vasodilator. The Human Plasma Kallikrein protein, when expressed in CHO (Chinese Hamster Ovary) cells, is often engineered with a His (histidine) tag to facilitate its purification and detection in biochemical studies. This recombinant Plasma Kallikrein-His fusion protein is commonly used in research related to coagulation disorders, inflammatory diseases, and therapeutic development.

      Synonyms

      Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein (PKK)

    • Uniprot ID

      P03952

      Sequence Note

      Gly20-Ala638

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant Human Plasma kallikrein.

    SDS-PAGE analysis of recombinant Human Plasma kallikrein.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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