Human P-selectin, His Tag, CHO

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Catalog Number

LDG054PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    CHO

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 85.81 kDa.
    The protein migrates as 93-140 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is <2 μg/mL.

    Form

    Lyophilized

• Background

  • Background

    Human P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It plays a critical role in the early stages of inflammation by mediating the interaction between leukocytes and the vascular endothelium, leading to leukocyte rolling and recruitment to sites of tissue injury or infection. The Human P-selectin protein, when expressed in CHO (Chinese Hamster Ovary) cells, can be tagged with a His (histidine) tag, which aids in its purification and detection for research purposes. This recombinant P-selectin-His fusion protein is widely used in studies focused on inflammation, thrombosis, and vascular biology, as well as in the development of anti-inflammatory therapies.

    Synonyms

    P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P

  • Uniprot ID

    P16109

    Sequence Note

    Trp42-Ala771

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human P-selectin, His Tag, CHO.
  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is <2 μg/mL.

  SDS-PAGE analysis of recombinant Human P-selectin.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.