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Leadgene Leadgene
Human Noggin, Fc Tag, HEK293
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Catalog Number

LDG017PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      HEK293 cell

    • Affinity Tag

      Human IgG1 Fc Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 49.11 kDa.
      The protein migrates as 58 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.05 µg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Noggin binds members of the transforming Growth Factors-beta (TGF beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP-4), which inactivates their activities. As a extracellular antagonist of BMP proteins, Noggin involves in the development of many body tissues, including nerve tissue, muscles, and bones. In addition, Noggin is able to inhibit chondrocyte differentiation through its interaction with GDF5.

      Uniprot ID

      Q13253

    • Sequence Note

      Gln28-Cys232

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human Noggin, Fc Tag, HEK293 (LDG017PHM), inhibits BMP-4-induced alkaline phosphatase production by ATDC5 cells, with an ED50 of &lt;0.05 µg/mL.

    Human Noggin, Fc Tag, HEK293 (LDG017PHM), inhibits BMP-4-induced alkaline phosphatase production by ATDC5 cells, with an ED50 of <0.05 µg/mL.

    SDS-PAGE analysis of recombinant human Noggin

    SDS-PAGE analysis of recombinant human Noggin

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  • Datasheet & Documents
    MSDS
    Manual
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