Human Nephrin, His-Avi Tag, CHO

Catalog Number

LDG200PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    CHO

  • Affinity Tag

    His-Avi Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 114.2 kDa.
    The protein migrates as 135-175 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Human Nephrin, encoded by NPHS1, is a key structural and signaling protein of the slit diaphragm between podocyte foot processes in the renal glomerulus. It plays an essential role in maintaining the glomerular filtration barrier and preventing proteinuria. Mutations or reduced expression of NPHS1 are associated with congenital nephrotic syndrome and other glomerular diseases. Nephrin also participates in podocyte cell adhesion and intracellular signaling pathways that regulate cytoskeletal organization and filtration barrier integrity, making it an important marker and research target in kidney disease, podocyte biology, and chronic kidney disease studies.

    Synonyms

    Nephrin, NPHS1, NPHN, Renal glomerulus-specific cell adhesion receptor, Slit diaphragm protein nephrin, Podocyte slit diaphragm protein, Nephrosis 1 protein

  • Uniprot ID

    O60500

    Sequence Note

    Gln23-Ser1055

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant Human Nephrin.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.