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Leadgene Leadgene
Human Midkine, His Tag, E. coli
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Catalog Number

LDG135PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 14.36 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Midkine, also known as neurite growth-promoting factor 2 (NEGF2) is a member of a small family of secreted Growth Factorss, furthermore high expression in lymph node, endometrium, spleen, and colon. Midkine is a 13.5 kDa protein containing 143 amino acids, which promotes angiogenesis, cell growth, migration, and gene expression of different cell types probably via a multiprotein receptor complex consisting of several molecules.

      Synonyms

      Midkine, Amphiregulin-associated protein, ARAP, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

    • Uniprot ID

      #P21741

      Sequence Note

      Val21-Asp143

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    The purity of recombinant human Midkine is greater than 95% as determined by SEC-HPLC.

    The purity of recombinant human Midkine is greater than 95% as determined by SEC-HPLC.

    SDS-PAGE analysis of recombinant human Midkine.

    SDS-PAGE analysis of recombinant human Midkine.

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  • Datasheet & Documents
    MSDS
    Manual
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