Human LR3 IGF-1, His Tag, E. coli

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Catalog Number

LDG194PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 9.9 kDa.
    The protein migrates as 10-11 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce MCF-7 cells proliferation in a serum-free condition. The ED50 for this effect is <2.5 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    LR3 IGF-1 (Long R3 Insulin-like Growth Factor-I) is a synthetic analog of IGF-1 engineered for increased potency and reduced binding to IGF-binding proteins. It stimulates cell proliferation and survival, and is widely used in cell culture and tissue engineering for enhanced growth effects.

    Synonyms

    Long-[Arg3]-Insulin-like Growth Factor 1, IGF-1 LR3, Long R3 IGF-I, IGF-I LR3, LR3 IGF-I

  • Uniprot ID

    P05019

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human LR3 IGF-1, His Tag, E. coli.
  Measure by its ability to induce MCF-7 cells proliferation in a serum-free condition. The ED50 for this effect is <2.5 ng/mL.

  SDS-PAGE analysis of recombinant human LR3 IGF-1 protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.