Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS containing 0.1% sarkosyl, pH 8.0.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 20.30 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce cytotoxicity in HT-29 cells in the presence of IFN-gamma. The ED₅₀ for this effect is < 10 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs). LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM). Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presenting anti-CD3 antibody treatment. Additionally, LIGHT can stimulate T cell proliferation and IFN-expression via interacting with TNFRS13 /HVEM.
LIGHT, Herpes virus entry mediator ligand, HVEM-L, CD258, Tumor necrosis factor ligand superfamily member 14
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
SDS-PAGE analysis of recombinant human LIGHT.1/1
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.