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Species of Origin
Human
Expression System
CHO
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>80% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 61.19 kDa.
The protein migrates as 65-70 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to bind human RSPO1 in functional ELISA. Immobilized Recombinant human RSPO1 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Human LGR5 Protein.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
LGR5 is a stem cell marker involved in Wnt signaling, regulating intestinal stem cell renewal and tumorigenesis in cancers.
Synonyms
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38
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Uniprot ID
O75473
Sequence Note
Gly 22 - Arg 561
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant Human LGR5]()
SDS-PAGE analysis of recombinant Human LGR5
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
