Human IL-8 (aa 28-99), His Tag, E. coli

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Catalog Number

LDG010PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 9.32 kDa.
    The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to chemoattract PBMC. The ED₅₀ for this effect is <2 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Human IL-8 (aa 28-99) predicts a molecular mass of 8.5 kDa and is the 72 amino acid residue form Ser 28 to Ser 99 of mature human IL-8. This recombinant protein is the major form secreted by macrophages. IL-8 is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells.

    Synonyms

    C-X-C motif chemokine 8, Chemokine, C-X-C motif ligand 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T-cell chemotactic factor

  • Uniprot ID

    #P10145

    Sequence Note

    Ser28-Ser99

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Measure by its ability to chemoattract PBMC using a concentration range of 1-10 ng/mL.
  Activity analysis of recombinant Human IL-8 (aa 28-99), His Tag, E. coli.

  SDS-PAGE analysis of recombinant human IL-8 (aa 28-99).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.