Human IL-6 ELISA Kit

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Catalog Number

LDG00025E

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• Overview

  Components

  Interleukin-6 (IL-6) ELISA plate Stripwell microplate	96 wells (12 x 8-well strips)
  Standard	2 vials (Lyophilized form)
  Standard reconstitution buffer	2 vials (1.1 mL)
  Standard & Sample diluent buffer	1 vial (12 mL)
  Assay buffer	1 vial (12 mL)
  HRP-antibody conjugate	1 vial (70 μL)
  HRP-antibody conjugated diluent buffer	1 vial (12 mL)
  20 X wash buffer	1 vial (15 mL)
  TMB	1 vial (12 mL)
  Stop solution	1 vial (6 mL)
  Microplate sealing film	1 sheet

  • Standard

    96 wells (12 x 8-well strips)

    2 vials (Lyophilized form)

  • Standard reconstitution buffer

    96 wells (12 x 8-well strips)

    2 vials (1.1 mL)

  • Standard & Sample diluent buffer

    96 wells (12 x 8-well strips)

    1 vial (12 mL)

  • Assay buffer

    96 wells (12 x 8-well strips)

    1 vial (12 mL)

  • HRP-antibody conjugate

    96 wells (12 x 8-well strips)

    1 vial (70 μL)

  • HRP-antibody conjugated diluent buffer

    96 wells (12 x 8-well strips)

    1 vial (12 mL)

  • 20 X wash buffer

    96 wells (12 x 8-well strips)

    1 vial (15 mL)

  • TMB

    96 wells (12 x 8-well strips)

    1 vial (12 mL)

  • Stop solution

    96 wells (12 x 8-well strips)

    1 vial (6 mL)

  • Microplate sealing film

    96 wells (12 x 8-well strips)

    1 sheet

• Specifications

  • Reactivity

    Human

    Application

    Sandwich ELISA analysis

  • Sensitivity

    0.0042 ng/mL

    Specificity

    Human IL-6

  • Assay Range

    0.015625 ng/mL-1 ng/mL

    Sample Type

    Human Serum / Human Plasma

  • Recovery

    Serum
    Stock: 73.4%
    2x dilution: 94.4%
    4x dilution: 103.6%
    8x dilution: 104.4%
    
    Plasma
    Stock: 82.6%
    2x dilution: 84%
    4x dilution: 90.2%
    8x dilution: 93.6%

• Background

  • Background

    Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8℃ for unopened product.

    Please refer to product manual for storage constructions.

• Image

  Typical data
  The following data are for demonstration only.

  An example of orientation of standards, blanks and samples in the stripwells microplate

  Reagents provided and reconstitution

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.