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Species of Origin
Human
Expression System
CHO
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Affinity Tag
Human IgG1 Fc Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>90% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated 72.87 MW of kDa.
The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by flow cytometry for its ability to bind a ligand expressed in A431 human epithelial carcinoma cell.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
IL-35 is an immunosuppressive cytokine composed of IL-12p35 and EBI3 subunits. It is involved in the suppression of immune responses and is studied for its roles in autoimmune diseases and cancer immunotherapy.
Synonyms
Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, NKSF
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Uniprot ID
Q14213
P29459Sequence Note
Arg21-Lys219
Arg23-Ser219
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant Human IL-35.]()
SDS-PAGE analysis of recombinant Human IL-35.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
