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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 18.93 kDa.
The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is <54 ng/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
More Images
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Specifications
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Background
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Background
Interleukin 33 (IL-33) is an 18 kDa cytokine with 169 amino acid residues. Interleukin-33 is a member of the IL-1 cytokine family and is primarily expressed in endothelial cells, epithelial cells, fibroblasts, and mesenchymal cells. When IL-33 binds to its receptor, ST2L, it activates mast cells and innate lymphoid cells and subsequently generates IL-5 and IL-13, which play essential roles in allergic inflammation. IL-33 also acts as an alarm by alerting the immune system of cellular damage and plays a critical role in type-2 innate immunity.
Synonyms
Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, Interleukin-33
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Uniprot ID
O95760
Sequence Note
Ser112-Thr270
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Activity analysis of recombinant Human IL-33, His Tag, E. coli. Measure by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is <54 ng/mL.]()
Activity analysis of recombinant Human IL-33, His Tag, E. coli.
Measure by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is <54 ng/mL.![SDS-PAGE analysis of recombinant human IL-33.]()
SDS-PAGE analysis of recombinant human IL-33.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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