Human IL-12 (p70), His Tag, HEK293

Catalog Number

LDG006PHM

Datasheet

More Documents

Loading package & price...

COA Search

• Specifications

  • Species of Origin

    Human

    Expression System

    HEK293

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 59.55 kDa.
    The protein migrates as 63-75 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC). The ED₅₀ for this effect is 0.05-0.2 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Interleukin 12 p70 (IL-12 p70) is an interleukin which is major secreted from immune cells like dendritic cells, macrophages and neutrophils. IL-12 p70 is a 58.5 kDa protein containing 527 amino acid residues which is composed by two subunits (IL-12 p35 and IL-12 p40). IL-12 p70 induces the cytotoxic activity of immune cells like NK cells and T cells. IL-12 p70 binds its receptor (IL-12Rβ1 and IL-12Rβ2) that activates the Jak/STAT signaling pathway. IL-12 p70 also plays an important role in cancer model which inhibits the formation of new blood vessels through the production of IFN γ.

    Synonyms

    Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2

  • Uniprot ID

    P29459
    P29460

    Sequence Note

    p35 : Arg 23 - Ser 219 (IL-12A)
    p40 : Ile 23 - Ser 328 (IL-12B)

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human IL-12 (p70).

  1

  /

  1

  Total   (1)

• Review

  

  Help others learn more about this product. Use the link below to share your experience.

  Submit a Review

• Publication

  

  There are currently no publications. Use the link below to let us know.

  Submit a Publication

• Datasheet & Documents

  Datasheet

  MSDS

  Manual

  1

  /

  1

Disclaimer：For Research Use or Further Manufacturing Only.