Leadgene Leadgene
Human IL-10, His Tag, HEK293
Catalog Number

LDG057PHM

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  • Specifications
    • Species of Origin

      Human

      Expression System

      HEK293

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.46 kDa.
      The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Human Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a pivotal role in limiting immune responses and preventing damage to the host during infections and autoimmune diseases. IL-10 is known for its ability to suppress the expression of pro-inflammatory cytokines, thereby regulating immune homeostasis. The Human IL-10 protein, when expressed in HEK293 (Human Embryonic Kidney 293) cells, is often tagged with a His (histidine) tag, which aids in its purification and detection during research studies. This recombinant IL-10-His fusion protein is widely utilized in research focused on immunology, inflammation, and therapeutic strategies for autoimmune and inflammatory diseases.

      Synonyms

      Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

    • Uniprot ID

      P22301

      Sequence Note

      Ser19-Asn178

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant Human IL-10 Protein.

    SDS-PAGE analysis of recombinant Human IL-10 Protein.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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