Leadgene Leadgene
Human IL-10 ELISA Kit
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  • Overview


    Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.

    Test Principle

    Human IL-10 ELISA kit is used to detect IL-10 in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human IL-10 monoclonal antibody to the solid phase. Human IL-10 in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human IL-10 monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity



      Sandwich ELISA analysis

    • Sensitivity

      0.00212 ng/mL


      Human Interleukin-10 (IL-10)

    • Assay Range

      0.039 ng/mL-25 ng/mL

      Sample Type

      Human Serum / Human Plasma

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    Total   (3)
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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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