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Leadgene Leadgene
Human IL-10 ELISA Kit
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Catalog Number

LDG00014E

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  • Overview

    Components

    Interleukin-10 (IL-10) ELISA plate

    96 wells (12 x 8-well strips)

    Standard

    2 vials (Lyophilized form)

    Standard reconstitution buffer

    1 vial (1.1 mL)

    Standard & Sample diluent buffer

    1 vial (12 mL)

    Assay buffer

    1 vial (12 mL)

    HRP-antibody conjugate

    1 vial (70 μL)

    HRP-antibody conjugated diluent buffer

    1 vial (12 mL)

    20 X wash buffer

    1 vial (15 mL)

    TMB

    1 vial (12 mL)

    Stop solution

    1 vial (6 mL)

    Microplate sealing film

    2 sheet

    • Standard

      96 wells (12 x 8-well strips)

      2 vials (Lyophilized form)

    • Standard reconstitution buffer

      96 wells (12 x 8-well strips)

      1 vial (1.1 mL)

    • Standard & Sample diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Assay buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • HRP-antibody conjugate

      96 wells (12 x 8-well strips)

      1 vial (70 μL)

    • HRP-antibody conjugated diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • 20 X wash buffer

      96 wells (12 x 8-well strips)

      1 vial (15 mL)

    • TMB

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Stop solution

      96 wells (12 x 8-well strips)

      1 vial (6 mL)

    • Microplate sealing film

      96 wells (12 x 8-well strips)

      2 sheet

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.00212 ng/mL

      Specificity

      Human Interleukin-10 (IL-10)

    • Assay Range

      0.039 ng/mL-25 ng/mL

      Sample Type

      Human Serum / Human Plasma

  • Background
    • Background

      Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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