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Leadgene Leadgene
Human IFN Gamma ELISA Kit
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Catalog Number

LDG00023E

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  • Overview

    Components

    Interferon-gamma (IFN-gamma) ELISA plate

    96 wells (12 x 8-well strips)

    Standard

    2 vials (Lyophilized form)

    Standard reconstitution buffer

    1 vial (1.1 mL)

    Standard & Sample diluent buffer

    2 vials (12 mL)

    HRP-antibody conjugate

    1 vial (70 μL)

    HRP-antibody conjugated diluent buffer

    1 vial (12 mL)

    20 X wash buffer

    1 vial (15 mL)

    TMB

    1 vial (12 mL)

    Stop solution

    1 vial (6 mL)

    Microplate sealing film

    2 sheets

    • Standard

      96 wells (12 x 8-well strips)

      2 vials (Lyophilized form)

    • Standard reconstitution buffer

      96 wells (12 x 8-well strips)

      1 vial (1.1 mL)

    • Standard & Sample diluent buffer

      96 wells (12 x 8-well strips)

      2 vials (12 mL)

    • HRP-antibody conjugate

      96 wells (12 x 8-well strips)

      1 vial (70 μL)

    • HRP-antibody conjugated diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • 20 X wash buffer

      96 wells (12 x 8-well strips)

      1 vial (15 mL)

    • TMB

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Stop solution

      96 wells (12 x 8-well strips)

      1 vial (6 mL)

    • Microplate sealing film

      96 wells (12 x 8-well strips)

      2 sheets

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.00606 ng/mL

      Specificity

      Human IFN Gamma

    • Assay Range

      0.015625 ng/mL-1 ng/mL

      Sample Type

      Human Serum / Human Plasma

    • Recovery

      Human Serum: 90-109%
      Human Plasma: 8X dilution: 83%

  • Background
    • Background

      The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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